Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM96038

EYS rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

EYS rabbit pAb from ELK Biotechnology Read more ›

EYS rabbit pAb 50µL/100µL
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EYS rabbit pAb 50µL/100µL

Product Description

EYS rabbit pAb is a research-use antibody from ELK Biotechnology for studying EYS in IHC, IF workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The product of this gene contains multiple epidermal growth factor (EGF)-like and LamG domains. The protein is expressed in the photoreceptor layer of the retina, and the gene is mutated in autosomal recessive retinitis pigmentosa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008],

Additional antibody information

Specificity This antibody detects endogenous levels of EYS at Human
Validation evidence captions Immunohistochemical analysis of paraffin-embedded human spleen tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200

Key Facts

Product typePrimary Antibody Target: EYS
Collaborator ELK Biotechnology
Partner model ES16647
Target EYS
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications IHC,IF
ConjugationUnconjugated
Dilution rangeIHC-p 1:50-200
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human EYS AA range: 2780-2830
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in retina (at protein level) (PubMed:18976725, PubMed:18836446, PubMed:27737822, PubMed:27846257). Isoform 1: Detected in retina (PubMed:27846257). Isoform 2: Detected in retina (PubMed:27846257). Isoform 3: Strongly expressed in retina and testis (PubMed:27846257). Isoform 4: Strongly expressed in testis, and weakly expressed in retina (PubMed:27846257).
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
Human IHC-p 1:50-200
Rat
Mouse
ABMIUM Validated™ IF
Species Dilution Notes

Specifications

Target
EYS
Concentration
1 mg/ml
Working concentration
IHC-p 1:50-200
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
IHCIF
Immunogen
Synthesized peptide derived from human EYS AA range: 2780-2830
Cellular localization
Cell projection, cilium, photoreceptor outer segment . Cell projection, cilium . Cytoplasm, cytoskeleton, cilium axoneme . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Secreted, extracellular space, extracellular matrix, interphotoreceptor matrix . Localizes to discrete puncta at, or adjacent to, the photoreceptor connecting cilium (PubMed:27737822). Highly expressed in cone photoreceptor outer segments (PubMed:27737822). Weakly expressed in rod photoreceptor outer segments (By similarity). May localize to the cilium axoneme (PubMed:27846257). May also be secreted into the interphotoreceptor extracellular matrix (PubMed:27737822). .
Dilution range
IHC-p 1:50-200
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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