FBW1B rabbit pAb
FBW1B rabbit pAb from ELK Biotechnology Read more ›
Product Description
FBW1B rabbit pAb is an ELK Biotechnology antibody for research workflows involving FBW1B.
Background
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats. This gene contains at least 14 exons, and its alternative splicing generates 3 transcript variants diverging at the presence/absence of two alternate exons. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | This antibody detects endogenous levels of FBW1B at Human/Mouse |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
| Target | FBW1B |
| Concentration | 1 mg/ml |
| Working concentration | WB 1：500-2000 |
| Species reactivity | Human; Mouse |
| Cellular localization | Cytoplasm . Nucleus . |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Oocyte meiosis;>>Ubiquitin mediated proteolysis;>>Cellular senescence;>>Wnt signaling pathway;>>Hedgehog signaling pathway;>>Hippo signaling pathway;>>Circadian rhythm;>>Shigellosis;>>Human immunodeficiency virus 1 infection |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1：500-2000 |
| Immunogen | Synthesized peptide derived from human FBW1B AA range: 464-514 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1：500-2000 | — |
| Mouse | — | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.