Amphiphysin I rabbit pAb from ELK Biotechnology Read more ›
Amphiphysin I rabbit pAb is a research-use antibody from ELK Biotechnology for studying Amphiphysin I in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Nov 2010],
| Specificity | Amphiphysin I Polyclonal Antibody detects endogenous levels of Amphiphysin I protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Amphiphysin I Polyclonal Antibody | Western Blot analysis of HeLa cells using Amphiphysin I Polyclonal Antibody | Western blot analysis of the lysates from HUVECcells using AMPH antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1647 |
| Target | Amphiphysin I |
| Research areas | >>Endocytosis, >>Fc gamma R-mediated phagocytosis |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AMPH. AA range:131-180 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Neurons, certain endocrine cell types and spermatocytes. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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