Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM95885

ALDH1B1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ALDH1B1 rabbit pAb from ELK Biotechnology Read more ›

ALDH1B1 rabbit pAb
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ALDH1B1 rabbit pAb

Product Description

ALDH1B1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ALDH1B1 in WB, ELISA workflows with listed reactivity for Human, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. This gene does not contain introns in the coding sequence. The variation of this locus may affect the development of alcohol-related problems. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity ALDH1B1 Polyclonal Antibody detects endogenous levels of ALDH1B1 protein.
Validation evidence captions Western Blot analysis of various cells using ALDH1B1 Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of COS7 cells using ALDH1B1 Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysates from HepG2 and COS cells, using ALDH1B1 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HepG2 cells using ALDH1B1 antibody.

Key Facts

Product typePrimary Antibody Target: ALDH1B1
Collaborator ELK Biotechnology
Partner model ES1637
Target ALDH1B1
Research areas>>Glycolysis / Gluconeogenesis, >>Ascorbate and aldarate metabolism, >>Fatty acid degradation, >>Valine, leucine and isoleucine degradation, >>Lysine degradation, >>Arginine and proline metabolism, >>Histidine metabolism, >>Tryptophan metabolism, >>beta-Alanine metabolism, >>Glycerolipid metabolism, >>Pyruvate metabolism, >>Pantothenate and CoA biosynthesis, >>Metabolic pathways, >>Biosynthesis of cofactors, >>Alcoholic liver disease
Reactivity Human,Monkey
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human ALDH1B1. AA range:311-360
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Liver, testis and to a lesser extent in brain.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Monkey
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Monkey
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
ALDH1B1
Also known as
ALDH1B1; ALDH5; ALDHX; Aldehyde dehydrogenase X; mitochondrial; Aldehyde dehydrogenase 5; Aldehyde dehydrogenase family 1 member B1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Monkey
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human ALDH1B1. AA range:311-360
Observed band
57kD
Cellular localization
Mitochondrion matrix.
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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