AKR1A1 rabbit pAb from ELK Biotechnology Read more ›
AKR1A1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying AKR1A1 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Multiple alternatively spliced transcript variants of this gene exist, all encoding the same protein. [provided by RefSeq, Jan 2011],
| Specificity | AKR1A1 Polyclonal Antibody detects endogenous levels of AKR1A1 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using AKR1A1 Polyclonal Antibody | Western Blot analysis of NIH-3T3 cells using AKR1A1 Polyclonal Antibody |
| Collaborator | ELK Biotechnology |
| Partner model | ES1624 |
| Target | AKR1A1 |
| Research areas | >>Glycolysis / Gluconeogenesis, >>Pentose and glucuronate interconversions, >>Ascorbate and aldarate metabolism, >>Glycerolipid metabolism, >>Pyruvate metabolism, >>Metabolic pathways, >>Biosynthesis of cofactors, >>Chemical carcinogenesis - reactive oxygen species |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from AKR1A1 . at AA range: 250-330 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Widely expressed. Highly expressed in kidney, salivary gland and liver. Detected in trachea, stomach, brain, lung, prostate, placenta, mammary gland, small intestine and lung. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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