Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM95777

GNG13 Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

GNG13 Rabbit pAb from ELK Biotechnology Read more ›

GNG13 Rabbit pAb 50µL/100µL
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GNG13 Rabbit pAb 50µL/100µL

Product Description

GNG13 Rabbit pAb is a research-use antibody from ELK Biotechnology for studying GNG13 in IHC, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Heterotrimeric G proteins, which consist of alpha (see MIM 139320), beta (see MIM 139380), and gamma subunits, function as signal transducers for the 7-transmembrane-helix G protein-coupled receptors. GNG13 is a gamma subunit that is expressed in taste, retinal, and neuronal tissues and plays a key role in taste transduction (Li et al., 2006 [PubMed 16473877]).[supplied by OMIM, Oct 2009],

Additional antibody information

Specificity This antibody detects endogenous levels of human GNG13
Validation evidence captions Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product typePrimary Antibody Target: GNG13
Collaborator ELK Biotechnology
Partner model ES16141
Target GNG13
Research areas>>Ras signaling pathway, >>Chemokine signaling pathway, >>PI3K-Akt signaling pathway, >>Apelin signaling pathway, >>Circadian entrainment, >>Retrograde endocannabinoid signaling, >>Glutamatergic synapse, >>Cholinergic synapse, >>Serotonergic synapse, >>GABAergic synapse, >>Dopaminergic synapse, >>Olfactory transduction, >>Taste transduction, >>Relaxin signaling pathway, >>Morphine addiction, >>Alcoholism, >>Human cytomegalovirus infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Human immunodeficiency virus 1 infection, >>Pathways in cancer
Reactivity Human,Mouse
Applications IHC,ELISA
ConjugationUnconjugated
Dilution rangeIHC-p 1:50-200, ELISA(peptide)1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human GNG13 AA range: 1-50
Concentration 1 mg/ml
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Brain,Cerebellum,
Purity The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
Human IHC-p 1:50-200
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
GNG13
Also known as
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-13
Concentration
1 mg/ml
Working concentration
IHC-p 1:50-200, ELISA(peptide)1:5000-20000
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
IHCELISA
Immunogen
Synthesized peptide derived from human GNG13 AA range: 1-50
Cellular localization
Cell membrane ; Lipid-anchor ; Cytoplasmic side .
Dilution range
IHC-p 1:50-200, ELISA(peptide)1:5000-20000
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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