GP-39 rabbit pAb
GP-39 rabbit pAb from ELK Biotechnology Read more ›
Product Description
GP-39 rabbit pAb is an ELK Biotechnology antibody for research workflows involving GP-39.
Background
Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009],
Additional antibody information
| Specificity | This antibody detects endogenous levels of human GP-39 |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°over night | Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). |
Key Facts
| Target | GP-39 |
| Also known as | Chitinase-3-like protein 1 (39 kDa synovial protein;Cartilage glycoprotein 39;CGP-39;GP-39;hCGP-39;YKL-40) |
| Concentration | 1 mg/ml |
| Working concentration | IHC-p 1:50-200, WB 1:500-2000 |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 42kD |
| Cellular localization | Secreted, extracellular space . Cytoplasm . Cytoplasm, perinuclear region . Endoplasmic reticulum . |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIF |
| Dilution range | IHC-p 1:50-200, WB 1:500-2000 |
| Immunogen | Synthesized peptide derived from human GP-39 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC-p 1:50-200 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.