Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM95735

GRAM2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

GRAM2 rabbit pAb from ELK Biotechnology Read more ›

GRAM2 rabbit pAb
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GRAM2 rabbit pAb

Product Description

GRAM2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying GRAM2 in WB workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Additional antibody information

Specificity This antibody detects endogenous levels of GRAM2 at Human/Mouse
Validation evidence captions Western blot analysis of lysates from 3T3 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: GRAM2
Collaborator ELK Biotechnology
Partner model ES15916
Target GRAM2
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human GRAM2 AA range: 150-200
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Brain,Hippocampus,
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse

Specifications

Target
GRAM2
Also known as
GRAM domain-containing protein 2
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human GRAM2 AA range: 150-200
Observed band
38kD
Cellular localization
Endoplasmic reticulum membrane ; Single-pass membrane protein . Cell membrane ; Peripheral membrane protein . Localizes to endoplasmic reticulum-plasma membrane contact sites (EPCS). Anchored at the ER, PM binding is mediated via GRAM domain and phosphatidylinositol lipid interaction. Localizes to distinct EPCS than GRAMD1A. .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.54% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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