Actin rabbit pAb from ELK Biotechnology Read more ›
Actin rabbit pAb is a research-use antibody from ELK Biotechnology for studying Actin pan in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Fish. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008],
| Specificity | Actin Polyclonal Antibody detects endogenous levels of Actin protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Actin Polyclonal Antibody diluted at 1:2000 | Western Blot analysis of HeLa cells using Actin Polyclonal Antibody diluted at 1:2000 | Western blot analysis of various lysis using Actin Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of various lysis using Actin Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
| Collaborator | ELK Biotechnology |
| Partner model | ES1589 |
| Target | Actin pan |
| Research areas | >>Rap1 signaling pathway, >>Phagosome, >>Apoptosis, >>Hippo signaling pathway, >>Focal adhesion, >>Adherens junction, >>Tight junction, >>Platelet activation, >>Neutrophil extracellular trap formation, >>Leukocyte transendothelial migration, >>Thermogenesis, >>Regulation of actin cytoskeleton, >>Thyroid hormone signaling pathway, >>Oxytocin signaling pathway, >>Gastric acid secretion, >>Amyotrophic lateral sclerosis, >>Bacterial invasion of epithelial cells, >>Vibrio cholerae infection, >>Pathogenic Escherichia coli infection, >>Shigellosis, >>Salmonella infection, >>Yersinia infection, >>Influenza A, >>Proteoglycans in cancer, >>Hepatocellular carcinoma, >>Hypertrophic cardiomyopathy, >>Arrhythmogenic right ventricular cardiomyopathy, >>Dilated cardiomyopathy, >>Viral myocarditis, >>Fluid shear stress and atherosclerosis |
| Reactivity | Human,Mouse,Rat,Fish |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Actin. AA range:326-375 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | B-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Fetal brain cortex,Foreskin,Hepatocellular car |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Fish |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Fish | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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