Acrp30 rabbit pAb from ELK Biotechnology Read more ›
Acrp30 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Acrp30 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
adiponectin, C1Q and collagen domain containing(ADIPOQ) Homo sapiens This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010],
| Specificity | Acrp30 Polyclonal Antibody detects endogenous levels of Acrp30 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from 1) HeLa, 2) VEC, 3) 293 cells, （Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. （Red） Tubulin β Monoclonal Antibody(5G3) (cat:YM3030) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:RS23710)was diluted at 1:10000, 37° 1hour. | Western Blot analysis of various cells using Acrp30 Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of HeLa cells using Acrp30 Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysate from HeLa cells, using Acrp30 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1586 |
| Target | Acrp30 |
| Research areas | >>PPAR signaling pathway, >>AMPK signaling pathway, >>Longevity regulating pathway, >>Adipocytokine signaling pathway, >>Type II diabetes mellitus, >>Non-alcoholic fatty liver disease, >>Alcoholic liver disease |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Acrp30. AA range:6-55 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Synthesized exclusively by adipocytes and secreted into plasma. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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