H2AB1 rabbit pAb
H2AB1 rabbit pAb from ELK Biotechnology Read more ›
Product Description
H2AB1 rabbit pAb is an ELK Biotechnology antibody for research workflows involving H2AB1.
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. This gene is part of a region that is repeated three times on chromosome X, once in intron 22 of the F8 gene and twice closer to the Xq telomere. This record represents the most centromeric copy which is in intron 22 of the F8 gene. [provided by RefSeq, Oct 2015],
Additional antibody information
| Specificity | This antibody detects endogenous levels of H2AB1 at Human/Mouse |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
| Target | H2AB1 |
| Concentration | 1 mg/ml |
| Working concentration | WB 1：500-2000 |
| Species reactivity | Human; Mouse |
| Cellular localization | Nucleus . Chromosome . Associated with the active X chromosome and with autosomes, while it is absent from the inactive X chromosome and excluded from Barr bodies. . |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Necroptosis;>>Neutrophil extracellular trap formation;>>Alcoholism;>>Systemic lupus erythematosus |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1：500-2000 |
| Immunogen | Synthesized peptide derived from human H2AB1 AA range: 49-99 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1：500-2000 | — |
| Mouse | — | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.