ABM95678
HEM6 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
HEM6 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
HEM6 rabbit pAb is an ELK Biotechnology antibody for research workflows involving HEM6.
Background
The protein encoded by this gene is the sixth enzyme of the heme biosynthetic pathway. The encoded enzyme is soluble and found in the intermembrane space of mitochondria. This enzyme catalyzes the stepwise oxidative decarboxylation of coproporphyrinogen III to protoporphyrinogen IX, a precursor of heme. Defects in this gene are a cause of hereditary coproporphyria (HCP).[provided by RefSeq, Oct 2009],
Additional antibody information
| Specificity | This antibody detects endogenous levels of HEM6 at Human/Mouse/Rat |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from PC-12 cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
Product type
Primary Antibody›
Target: HEM6
| Target | HEM6 |
| Concentration | 1 mg/ml |
| Working concentration | WB 1：500-2000 |
| Species reactivity | Human; Mouse;Rat |
| Cellular localization | Mitochondrion intermembrane space. |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Porphyrin metabolism;>>Metabolic pathways;>>Biosynthesis of cofactors |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1：500-2000 |
| Immunogen | Synthesized peptide derived from human HEM6 AA range: 265-315 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1：500-2000 | — |
| Mouse | — | — |
| Rat | — | — |
Specifications
Target
HEM6
Concentration
1 mg/ml
Working concentration
WB 1：500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human; Mouse;Rat
Tested applications
WB
Immunogen
Synthesized peptide derived from human HEM6 AA range: 265-315
Cellular localization
Mitochondrion intermembrane space.
Dilution range
WB 1：500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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