ABM95675
HERC3 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
HERC3 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
HERC3 rabbit pAb is an ELK Biotechnology antibody for research workflows involving HERC3.
Background
This gene encodes a member the HERC ubiquitin ligase family. The encoded protein is located in the cytosol and binds ubiquitin via a HECT domain. Mutations in this gene have been associated with colorectal and gastric carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2012],
Additional antibody information
| Specificity | This antibody detects endogenous levels of HERC3 at Human |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from 3T3 cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
Product type
Primary Antibody›
Target: HERC3
| Target | HERC3 |
| Concentration | 1 mg/ml |
| Working concentration | WB 1：500-2000 |
| Species reactivity | Human;Rat;Mouse; |
| Cellular localization | Cytoplasm. Cytoplasmic vesicle. Also found in vesicular-like structures. |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Ubiquitin mediated proteolysis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1：500-2000 |
| Immunogen | Synthesized peptide derived from human HERC3 AA range: 985-1035 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1：500-2000 | — |
| Rat | — | — |
| Mouse | — | — |
Specifications
Target
HERC3
Concentration
1 mg/ml
Working concentration
WB 1：500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WB
Immunogen
Synthesized peptide derived from human HERC3 AA range: 985-1035
Cellular localization
Cytoplasm. Cytoplasmic vesicle. Also found in vesicular-like structures.
Dilution range
WB 1：500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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