Manufactured by:ELK Biotechnology
RUO

Jak1(phospho-Tyr1034/1035) rabbit pAb

Jak1(phospho-Tyr1034/1035) rabbit pAb from ELK Biotechnology

SKU:
ABM105433
Product type
Primary Antibody
TargetJAK1
ApplicationsWB, IHC
Species / reactivityHuman, Mouse, Rat
Expression systemExpressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
PurityThe antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Jak1(phospho-Tyr1034/1035) rabbit pAb is a research-use antibody from ELK Biotechnology for studying JAK1 in WB, IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016],

Additional antibody information

Specificity This antibody detects endogenous levels of Human Mouse Rat Jak1(phospho-Tyr1034 or 1035)
Validation evidence captions Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
Key facts & specifications
Research areas
>>EGFR tyrosine kinase inhibitor resistance, >>PI3K-Akt signaling pathway, >>Necroptosis, >>Osteoclast differentiation, >>Signaling pathways regulating pluripotency of stem cells, >>NOD-like receptor signaling pathway, >>JAK-STAT signaling pathway, >>Th1 and Th2 cell differentiation, >>Th17 cell differentiation, >>Leishmaniasis, >>Toxoplasmosis, >>Tuberculosis, >>Hepatitis C, >>Hepatitis B, >>Measles, >>Human cytomegalovirus infection, >>Influenza A, >>Human papillomavirus infection, >>Human T-cell leukemia virus 1 infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Herpes simplex virus 1 infection, >>Epstein-Barr virus infection, >>Coronavirus disease - COVID-19, >>Pathways in cancer, >>Viral carcinogenesis, >>Pancreatic cancer, >>PD-L1 expression and PD-1 checkpoint pathway in cancer
Also known as
Tyrosine-protein kinase JAK1 (EC 2.7.10.2) (Janus kinase 1) (JAK-1)
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Immunogen
Synthesized phosho peptide around human Jak1(Tyr1034 and 1035)
Observed band
132kD
Cellular localization
Endomembrane system; Peripheral membrane protein. Wholly intracellular, possibly membrane associated.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES15397
UniProt entry
P23458
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

JAK1Janus kinase 1

View target guide

JAK1 identifies Janus kinase 1 in Homo sapiens (Human). This target record connects NCBI Gene 3716, UniProtKB P23458 and related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
NCBI Gene
3716
UniProtKB
P23458
ELK Biotechnology
£220.00