Tensin-1 (phospho Tyr1326) rabbit pAb from ELK Biotechnology Read more ›
Tensin-1 (phospho Tyr1326) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Tensin-1 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene localizes to focal adhesions, regions of the plasma membrane where the cell attaches to the extracellular matrix. This protein crosslinks actin filaments and contains a Src homology 2 (SH2) domain, which is often found in molecules involved in signal transduction. This protein is a substrate of calpain II. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015],
| Specificity | Phospho-Tensin-1 (Y1326) Polyclonal Antibody detects endogenous levels of Tensin-1 protein only when phosphorylated at Y1326. |
|---|---|
| Validation evidence captions | Western Blot analysis of extracts from K562 cells, using Phospho-Tensin-1 (Y1326) Polyclonal Antibody. | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Tensin-1 (Phospho-Tyr1326) Antibody | Western blot analysis of lysates from A10 cells, using phospho-Thrensin-1 (Phospho-Tyr1326) antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1531 |
| Target | Tensin-1 |
| Research areas | Antibody Research |
| Reactivity | Human,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human Tensin-1 (phospho Tyr1326) |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Ubiquitous. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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