ABM95438
LCN12 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
LCN12 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
LCN12 rabbit pAb is an ELK Biotechnology antibody for research workflows involving LCN12.
Background
Members of the lipocalin family, such as LCN12, have a common structure consisting of an 8-stranded antiparallel beta-barrel that forms a cup-shaped ligand-binding pocket or calyx. Lipocalins generally bind small hydrophobic ligands and transport them to specific cells (Suzuki et al., 2004 [PubMed 15363845]).[supplied by OMIM, Aug 2009],
Additional antibody information
| Specificity | This antibody detects endogenous levels of LCN12 at Human/Mouse/Rat |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°over night | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
Product type
Primary Antibody›
Target: LCN12
| Target | LCN12 |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Species reactivity | Human; Mouse;Rat |
| Cellular localization | Secreted . |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCELISA |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Immunogen | Synthesized peptide derived from human LCN12 AA range: 56-106 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
LCN12
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human; Mouse;Rat
Tested applications
WBIHCELISA
Immunogen
Synthesized peptide derived from human LCN12 AA range: 56-106
Cellular localization
Secreted .
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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