Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM95344

MAFA rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

MAFA rabbit pAb from ELK Biotechnology Read more ›

MAFA rabbit pAb
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MAFA rabbit pAb

Product Description

MAFA rabbit pAb is a research-use antibody from ELK Biotechnology for studying MAFA in WB workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).[supplied by OMIM, Mar 2008],

Additional antibody information

Specificity This antibody detects endogenous levels of MAFA at Human/Mouse
Validation evidence captions Western blot analysis of lysates from MDA-MB cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: MAFA
Collaborator ELK Biotechnology
Partner model ES15057
Target MAFA
Research areas>>Type II diabetes mellitus, >>Maturity onset diabetes of the young
Reactivity Human,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human MAFA AA range: 204-254
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in the islets of Langerhans (at protein level).
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse

Specifications

Target
MAFA
Also known as
Transcription factor MafA (Pancreatic beta-cell-specific transcriptional activator) (Transcription factor RIPE3b1) (V-maf musculoaponeurotic fibrosarcoma oncogene homolog A)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human MAFA AA range: 204-254
Observed band
38kD
Cellular localization
Nucleus .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.36% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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