Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM95309

MER34 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

MER34 rabbit pAb from ELK Biotechnology Read more ›

MER34 rabbit pAb
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MER34 rabbit pAb

Product Description

MER34 rabbit pAb is a research-use antibody from ELK Biotechnology for studying MER34 in WB workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Additional antibody information

Specificity This antibody detects endogenous levels of MER34 at Human
Validation evidence captions Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: MER34
Collaborator ELK Biotechnology
Partner model ES14974
Target MER34
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human MER34 AA range: 391-441
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Expressed at high level in the placenta and stem cells (at protein level) (PubMed:28739914). Also expressed in the kidney but at a lower level (PubMed:28739914). Endogenous retroviral envelope protein HEMO, secreted form: Present in the blood of pregnant women (at protein level) (PubMed:28739914).
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Rat
Mouse

Specifications

Target
MER34
Also known as
HERV-MER_4q12 provirus ancestral Env polyprotein (Endogenous retrovirus group MER34 member 1)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human MER34 AA range: 391-441
Observed band
60kD
Cellular localization
Endogenous retroviral envelope protein HEMO: Cell membrane ; Single-pass type I membrane protein . This uncleaved form present at the cell surface represents the major form (PubMed:28739914). .; Endogenous retroviral envelope protein HEMO, secreted form: Secreted . This secreted form, which is released in the extracellular medium following cleavage, constitutes a minor form (PubMed:28739914). .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.109% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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