ABM95271
FAK (phospho Tyr397) rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
FAK (phospho Tyr397) rabbit pAb from ELK Biotechnology Read more ›
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Product Description
FAK (phospho Tyr397) rabbit pAb is an ELK Biotechnology antibody for research workflows involving FAK.
Background
protein tyrosine kinase 2(PTK2) Homo sapiens This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only four of them have been determined. [provided by RefSeq, Oct 2015],
Additional antibody information
| Specificity | Phospho-FAK (Y397) Polyclonal Antibody detects endogenous levels of FAK protein only when phosphorylated at Y397. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from 1) 293T, 2) AD293 ,3) Hela cells, （Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. （Red） Actin β Monoclonal Antibody(5B7) (cat:YM3028) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:RS23710)was diluted at 1:10000, 37° 1hour. | Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,FAK (phospho Tyr397) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. | Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,FAK (phospho Tyr397) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. | Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1,FAK (phospho Tyr397) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
Key Facts
Product type
Primary Antibody›
Target: FAK
| Target | FAK |
| Also known as | PTK2; FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 119kD |
| Cellular localization | Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Nucleus. Cytoplasm, cytoskeleton, cilium basal body . Constituent of focal adhesions. Detected at microtubules. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Endocrine resistance;>>ErbB signaling pathway;>>Chemokine signaling pathway;>>PI3K-Akt signaling pathway;>>Axon guidance;>>VEGF signaling pathway;>>Focal adhesion;>>Leukocyte transendothelial migration;>>Regulation of actin cytoskeleton;>>Growth hormone synthesis, secretion and action;>>Bacterial invasion of epithelial cells;>>Shigellosis;>>Yersinia infection;>>Amoebiasis;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Human immunodeficiency virus 1 infection;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Proteoglycans in cancer;>>Chemical carcinogenesis - reactive oxygen species;>>Small cell lung cancer;>>Lipid and atherosclerosis;>>Fluid shear stress and atherosclerosis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FAK around the phosphorylation site of Tyr397. AA range:363-412 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
FAK
Also known as
PTK2; FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human FAK around the phosphorylation site of Tyr397. AA range:363-412
Observed band
119kD
Cellular localization
Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Nucleus. Cytoplasm, cytoskeleton, cilium basal body . Constituent of focal adhesions. Detected at microtubules.
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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