ABM95234
Caspase-9 (phospho Thr125) rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Caspase-9 (phospho Thr125) rabbit pAb from ELK Biotechnology Read more ›
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Product Description
Caspase-9 (phospho Thr125) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Caspase-9.
Background
CASP9 encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Caspase 9 can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. Caspase 9 is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
Additional antibody information
| Specificity | Phospho-Caspase-9 (T125) Polyclonal Antibody detects endogenous levels of Caspase-9 protein only when phosphorylated at T125. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-Caspase-9 (T125) Polyclonal Antibody diluted at 1:500 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Caspase 9 (Phospho-Thr125) Antibody | Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Caspase 9 (Phospho-Thr125) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from HeLa cells treated with TNF 20ng/ml 5'+calyculinA 50ng/ml 5', using Caspase 9 (Phospho-Thr125) Antibody. The lane on the right is blocked with the phospho peptide. |
Key Facts
Product type
Primary Antibody›
Target: Caspase-9
| Target | Caspase-9 |
| Also known as | CASP9; MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 46kD |
| Cellular localization | nucleus,mitochondrion,cytosol,apoptosome, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Platinum drug resistance;>>p53 signaling pathway;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Apoptosis - multiple species;>>VEGF signaling pathway;>>Thyroid hormone signaling pathway;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Pathogenic Escherichia coli infection;>>Legionellosis;>>Toxoplasmosis;>>Tuberculosis;>>Hepatitis C;>>Hepatitis B;>>Measles;>>Human cytomegalovirus infection;>>Influenza A;>>Kaposi sarcoma-associated herpesvirus infection;>>Herpes simplex virus 1 infection;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection;>>Pathways in cancer;>>Colorectal cancer;>>Pancreatic cancer;>>Endometrial cancer;>>Prostate cancer;>>Small cell lung cancer;>>Non-small cell lung cancer;>>Viral myocarditis;>>Lipid and atherosclerosis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Caspase 9 around the phosphorylation site of Thr125. AA range:91-140 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
Caspase-9
Also known as
CASP9; MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human Caspase 9 around the phosphorylation site of Thr125. AA range:91-140
Observed band
46kD
Cellular localization
nucleus,mitochondrion,cytosol,apoptosome,
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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