NFκB-p105/p50 (phospho Ser337) rabbit pAb from ELK Biotechnology Read more ›
NFκB-p105/p50 (phospho Ser337) rabbit pAb is a research-use antibody from ELK Biotechnology for studying NFKB1 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
nuclear factor kappa B subunit 1(NFKB1) Homo sapiens This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isof
| Specificity | Phospho-NFκB-p105/p50 (S337) Polyclonal Antibody detects endogenous levels of NFκB-p105/p50 protein only when phosphorylated at S337. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-NFκB-p105/p50 (S337) Polyclonal Antibody diluted at 1:500 | Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Immunohistochemistry analysis of paraffin-embedded human breast cancer, using NF-κB p105/p50 (Phospho-Ser337) Antibody. The picture on the right is blocked with the NF-κB p105/p50 (Phospho-Ser337) peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1466 |
| Target | NFKB1 |
| Research areas | >>Antifolate resistance, >>MAPK signaling pathway, >>Ras signaling pathway, >>cAMP signaling pathway, >>Chemokine signaling pathway, >>NF-kappa B signaling pathway, >>HIF-1 signaling pathway, >>Sphingolipid signaling pathway, >>PI3K-Akt signaling pathway, >>Apoptosis, >>Longevity regulating pathway, >>Cellular senescence, >>Osteoclast differentiation, >>Neutrophil extracellular trap formation, >>Toll-like receptor signaling pathway, >>NOD-like receptor signaling pathway, >>RIG-I-like receptor signaling pathway, >>Cytosolic DNA-sensing pathway, >>C-type lectin receptor signaling pathway, >>IL-17 signaling pathway, >>Th1 and Th2 cell differentiation, >>Th17 cell differentiation, >>T cell receptor signaling pathway, >>B cell receptor signaling pathway, >>TNF signaling pathway, >>Neurotrophin signaling pathway, >>Prolactin signaling pathway, >>Adipocytokine signaling pathway, >>Relaxin signaling pathway, >>Insulin resistance, >>Non-alcoholic fatty liver disease, >>AGE-RAGE signaling pathway in diabetic complications, >>A |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human NFκB-p105/p50 (phospho Ser337) |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Muscle,Rectum tumor,Uterus, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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