Synaptotagmin 1/2 (phospho Thr202/199) rabbit pAb from ELK Biotechnology Read more ›
Synaptotagmin 1/2 (phospho Thr202/199) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Synaptotagmin 1/2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010],
| Specificity | Phospho-Synaptotagmin 1/2 (T202/199) Polyclonal Antibody detects endogenous levels of Synaptotagmin 1/2 protein only when phosphorylated at T202/199. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-Synaptotagmin 1/2 (T202/199) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Synaptotagmin (Phospho-Thr202) Antibody | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Synaptotagmin (Phospho-Thr202) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from 293 cells treated with Forskolin 40nM 30', using Synaptotagmin (Phospho-Thr202) Antibody. The lane on the left is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1461 |
| Target | Synaptotagmin 1/2 |
| Research areas | >>Synaptic vesicle cycle |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Synaptotagmin around the phosphorylation site of Thr202. AA range:176-225 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in melanocytes (PubMed:23999003). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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