Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM95183

NCK5L rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

NCK5L rabbit pAb from ELK Biotechnology Read more ›

NCK5L rabbit pAb
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NCK5L rabbit pAb

Product Description

NCK5L rabbit pAb is a research-use antibody from ELK Biotechnology for studying NCK5L in WB workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Additional antibody information

Specificity This antibody detects endogenous levels of NCK5L at Human/Mouse
Validation evidence captions Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: NCK5L
Collaborator ELK Biotechnology
Partner model ES14542
Target NCK5L
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human NCK5L AA range: 261-311
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse

Specifications

Target
NCK5L
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human NCK5L AA range: 261-311
Cellular localization
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Localizes to microtubule plus ends (PubMed:26485573, PubMed:26482847). Associates with centrosomes during interphase, but dissociates from these structures from the onset of mitosis (PubMed:26549230). .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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