Shc (phospho Tyr427) rabbit pAb from ELK Biotechnology Read more ›
Shc (phospho Tyr427) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Shc in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011],
| Specificity | Phospho-Shc (Y427) Polyclonal Antibody detects endogenous levels of Shc protein only when phosphorylated at Y427. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-Shc (Y427) Polyclonal Antibody diluted at 1:500 | Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Immunohistochemistry analysis of paraffin-embedded human brain, using Shc (Phospho-Tyr427) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 5', using Shc (Phospho-Tyr427) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1454 |
| Target | Shc |
| Research areas | >>EGFR tyrosine kinase inhibitor resistance, >>Endocrine resistance, >>ErbB signaling pathway, >>Ras signaling pathway, >>Chemokine signaling pathway, >>Phospholipase D signaling pathway, >>Focal adhesion, >>Natural killer cell mediated cytotoxicity, >>Neurotrophin signaling pathway, >>Insulin signaling pathway, >>Estrogen signaling pathway, >>Prolactin signaling pathway, >>Relaxin signaling pathway, >>Growth hormone synthesis, secretion and action, >>Alcoholism, >>Bacterial invasion of epithelial cells, >>MicroRNAs in cancer, >>Glioma, >>Chronic myeloid leukemia, >>Breast cancer, >>Hepatocellular carcinoma, >>Gastric cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Shc around the phosphorylation site of Tyr427. AA range:393-442 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Widely expressed. Expressed in neural stem cells but absent in mature neurons. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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