NDUFA1 Rabbit pAb from ELK Biotechnology Read more ›
NDUFA1 Rabbit pAb is a research-use antibody from ELK Biotechnology for studying NDUFA1 in IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However, the NDUFA1 peptide is one of about 31 components of the "hydrophobic protein" (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function. [provided by RefSeq, Jul 2008],
| Specificity | This antibody detects endogenous levels of human NDUFA1 |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES14533 |
| Target | NDUFA1 |
| Research areas | >>Oxidative phosphorylation, >>Metabolic pathways, >>Thermogenesis, >>Retrograde endocannabinoid signaling, >>Non-alcoholic fatty liver disease, >>Alzheimer disease, >>Parkinson disease, >>Amyotrophic lateral sclerosis, >>Huntington disease, >>Prion disease, >>Pathways of neurodegeneration - multiple diseases, >>Chemical carcinogenesis - reactive oxygen species, >>Diabetic cardiomyopathy |
| Reactivity | Human,Mouse,Rat |
| Applications | IHC,ELISA |
| Conjugation | Unconjugated |
| Dilution range | IHC-p 1:50-200, ELISA(peptide)1:5000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from human NDUFA1 AA range: 20-100 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Primarily expressed in heart and skeletal muscle. |
| Purity | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC-p 1:50-200 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
