GABAA Rβ1 (phospho Ser434) rabbit pAb from ELK Biotechnology Read more ›
GABAA Rβ1 (phospho Ser434) rabbit pAb is a research-use antibody from ELK Biotechnology for studying GABAA Rβ1 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
The gamma-aminobutyric acid (GABA) A receptor is a multisubunit chloride channel that mediates the fastest inhibitory synaptic transmission in the central nervous system. This gene encodes GABA A receptor, beta 1 subunit. It is mapped to chromosome 4p12 in a cluster comprised of genes encoding alpha 4, alpha 2 and gamma 1 subunits of the GABA A receptor. Alteration of this gene is implicated in the pathogenetics of schizophrenia. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-GABAA Rβ1 (S434) Polyclonal Antibody detects endogenous levels of GABAA Rβ1 protein only when phosphorylated at S434. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-GABAA Rβ1 (S434) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using GABA-RB (Phospho-Ser434) Antibody | Immunohistochemistry analysis of paraffin-embedded human brain, using GABA-RB (Phospho-Ser434) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from COS7 cells, using GABA-RB (Phospho-Ser434) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1452 |
| Target | GABAA Rβ1 |
| Research areas | >>Neuroactive ligand-receptor interaction, >>Retrograde endocannabinoid signaling, >>Serotonergic synapse, >>GABAergic synapse, >>Morphine addiction, >>Nicotine addiction |
| Reactivity | Human,Mouse,Rat,Monkey |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GABA-RB around the phosphorylation site of Ser434. AA range:401-450 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Epithelium, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Monkey | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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