Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM95101

NUP54 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

NUP54 rabbit pAb from ELK Biotechnology Read more ›

NUP54 rabbit pAb
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NUP54 rabbit pAb

Product Description

NUP54 rabbit pAb is a research-use antibody from ELK Biotechnology for studying NUP54 in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. This gene encodes a member of the phe-gly (FG) repeat-containing nucleoporin subset. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013],

Additional antibody information

Specificity This antibody detects endogenous levels of NUP54 at Human/Mouse/Rat
Validation evidence captions Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: NUP54
Collaborator ELK Biotechnology
Partner model ES14424
Target NUP54
Research areas>>Nucleocytoplasmic transport, >>Amyotrophic lateral sclerosis
Reactivity Human,Mouse,Rat
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human NUP54 AA range: 98-148
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse
Rat

Specifications

Target
NUP54
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WB
Immunogen
Synthesized peptide derived from human NUP54 AA range: 98-148
Cellular localization
Nucleus, nuclear pore complex . Nucleus membrane ; Peripheral membrane protein ; Cytoplasmic side . Nucleus membrane ; Peripheral membrane protein ; Nucleoplasmic side . Biased towards cytoplasmic side. Central region of the nuclear pore complex, within the transporter. .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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