Caspase-8 (phospho Tyr380) rabbit pAb from ELK Biotechnology Read more ›
Caspase-8 (phospho Tyr380) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Caspase-8 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alt
| Specificity | Phospho-Caspase-8 (Y380) Polyclonal Antibody detects endogenous levels of Caspase-8 protein only when phosphorylated at Y380. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-Caspase-8 (Y380) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Caspase 8 (Phospho-Tyr380) Antibody | Western blot analysis of lysates from LOVO cells treated with UV 15', using Caspase 8 (Phospho-Tyr380) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1437 |
| Target | Caspase-8 |
| Research areas | >>Platinum drug resistance, >>p53 signaling pathway, >>Apoptosis, >>Apoptosis - multiple species, >>Necroptosis, >>Toll-like receptor signaling pathway, >>NOD-like receptor signaling pathway, >>RIG-I-like receptor signaling pathway, >>C-type lectin receptor signaling pathway, >>IL-17 signaling pathway, >>TNF signaling pathway, >>Non-alcoholic fatty liver disease, >>Alcoholic liver disease, >>Alzheimer disease, >>Huntington disease, >>Pathways of neurodegeneration - multiple diseases, >>Pathogenic Escherichia coli infection, >>Salmonella infection, >>Legionellosis, >>Chagas disease, >>Toxoplasmosis, >>Tuberculosis, >>Hepatitis C, >>Hepatitis B, >>Measles, >>Human cytomegalovirus infection, >>Influenza A, >>Human papillomavirus infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Herpes simplex virus 1 infection, >>Epstein-Barr virus infection, >>Human immunodeficiency virus 1 infection, >>Pathways in cancer, >>Viral carcinogenesis, >>Viral myocarditis, >>Lipid and atherosclerosis |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Caspase 8 around the phosphorylation site of Tyr380. AA range:346-395 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
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For research applications. Not for diagnostic use.
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