Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM95060

OSBL8 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

OSBL8 rabbit pAb from ELK Biotechnology Read more ›

OSBL8 rabbit pAb
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OSBL8 rabbit pAb

Product Description

OSBL8 rabbit pAb is a research-use antibody from ELK Biotechnology for studying OSBL8 in WB workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of a family of proteins containing an N-terminal pleckstrin homology domain and a highly conserved C-terminal oxysterol-binding protein-like sterol-binding domain. It binds mutliple lipid-containing molecules, including phosphatidylserine, phosphatidylinositol 4-phosphate (PI4P) and oxysterol, and promotes their exchange between the endoplasmic reticulum and the plasma membrane. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016],

Additional antibody information

Specificity This antibody detects endogenous levels of OSBL8 at Human
Validation evidence captions Western blot analysis of lysates from CACO2 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: OSBL8
Collaborator ELK Biotechnology
Partner model ES14365
Target OSBL8
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human OSBL8 AA range: 290-340
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Widely expressed (PubMed:11735225). Expressed at higher level in macrophages (PubMed:17991739).
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Rat
Mouse

Specifications

Target
OSBL8
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human OSBL8 AA range: 290-340
Cellular localization
Isoform 1: Endoplasmic reticulum membrane ; Single-pass membrane protein . Nucleus membrane . The presence of the N-terminus extension contains an overall negative charge that may explain the weak localization to the cortical endoplasmic reticulum (Probable). .; Isoform 3: Endoplasmic reticulum membrane ; Single-pass membrane protein. Localizes to the cortical endoplasmic reticulum at the endoplasmic reticulum-plasma membrane contact sites. .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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