p70 S6 Kinase (phospho-Thr389) rabbit pAb from ELK Biotechnology Read more ›
p70 S6 Kinase (phospho-Thr389) rabbit pAb is a research-use antibody from ELK Biotechnology for studying p70 S6 kinase α in WB workflows with listed reactivity for Human, Mouse, Rat, Bovine. Specifications and supporting documents below should be reviewed when planning the experiment.
ribosomal protein S6 kinase B1(RPS6KB1) Homo sapiens This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013],
| Specificity | This antibody detects endogenous levels of Human Mouse Rat p70 S6 Kinase (phospho-Thr389) |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). | Western blot analysis of lysates from Hela cells, treated with LPS 100ng/ml 30', using total P70 S6 Kinase Antibody(catalog: YT3555) and P70 S6 Kinase (phospho T389) antibody(catalog: YP1427).(Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. |
| Collaborator | ELK Biotechnology |
| Partner model | ES14291 |
| Target | p70 S6 kinase α |
| Research areas | >>EGFR tyrosine kinase inhibitor resistance, >>Endocrine resistance, >>ErbB signaling pathway, >>HIF-1 signaling pathway, >>Autophagy - animal, >>mTOR signaling pathway, >>PI3K-Akt signaling pathway, >>AMPK signaling pathway, >>Longevity regulating pathway, >>Longevity regulating pathway - multiple species, >>TGF-beta signaling pathway, >>Apelin signaling pathway, >>Fc gamma R-mediated phagocytosis, >>Thermogenesis, >>Insulin signaling pathway, >>Insulin resistance, >>Shigellosis, >>Human cytomegalovirus infection, >>Human papillomavirus infection, >>Human immunodeficiency virus 1 infection, >>Pathways in cancer, >>Proteoglycans in cancer, >>Chemical carcinogenesis - receptor activation, >>Colorectal cancer, >>Pancreatic cancer, >>Acute myeloid leukemia, >>Breast cancer, >>Hepatocellular carcinoma, >>Gastric cancer, >>Choline metabolism in cancer, >>PD-L1 expression and PD-1 checkpoint pathway in cancer |
| Reactivity | Human,Mouse,Rat,Bovine |
| Applications | WB |
| Conjugation | Unconjugated |
| Dilution range | WB 1:1000-2000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized phosho peptide around human p70 S6 Kinase (Thr389) |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Widely expressed. |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Bovine |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1000-2000 | — |
| Mouse | — | — |
| Rat | — | — |
| Bovine | — | — |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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