Btk (phospho Tyr223) rabbit pAb from ELK Biotechnology Read more ›
Btk (phospho Tyr223) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Btk in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013],
| Specificity | Phospho-Btk (Y223) Polyclonal Antibody detects endogenous levels of Btk protein only when phosphorylated at Y223. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-Btk (Y223) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using BTK (Phospho-Tyr223) Antibody | Western blot analysis of lysates from HeLa cells treated with Serum 10% 15', using BTK (Phospho-Tyr223) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1422 |
| Target | Btk |
| Research areas | >>NF-kappa B signaling pathway, >>Osteoclast differentiation, >>Platelet activation, >>B cell receptor signaling pathway, >>Fc epsilon RI signaling pathway, >>Epstein-Barr virus infection, >>Primary immunodeficiency |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human BTK around the phosphorylation site of Tyr223. AA range:188-237 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Predominantly expressed in B-lymphocytes. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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