PCSK6 rabbit pAb
PCSK6 rabbit pAb from ELK Biotechnology Read more ›
Product Description
PCSK6 rabbit pAb is an ELK Biotechnology antibody for research workflows involving PCSK6.
Background
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to the trans-Golgi network where a second autocatalytic event takes place and the catalytic activity is acquired. The encoded protease is constitutively secreted into the extracellular matrix and expressed in many tissues, including neuroendocrine, liver, gut, and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include transforming growth factor beta related proteins, proalbumin, and von Willebrand factor. This gene is thought to play a role in tumor progression and left-right patterning. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2014],
Additional antibody information
| Specificity | This antibody detects endogenous levels of PCSK6 at Human/Rat |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from 3T3 cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
| Target | PCSK6 |
| Also known as | Proprotein convertase subtilisin/kexin type 6 (EC 3.4.21.-) (Paired basic amino acid cleaving enzyme 4) (Subtilisin-like proprotein convertase 4) (SPC4) (Subtilisin/kexin-like protease PACE4) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000 |
| Species reactivity | Human;Rat |
| Observed band | 105kD |
| Cellular localization | Isoform PACE4A-I: Secreted.; Isoform PACE4A-II: Secreted.; Isoform PACE4C: Endoplasmic reticulum. Not secreted, remains probably in zymogen form in endoplasmic reticulum.; Isoform PACE4CS: Endoplasmic reticulum. Not secreted, remains probably in zymogen form in endoplasmic reticulum.; Isoform PACE4E-I: Endomembrane system; Peripheral membrane protein. Retained intracellularly probably through a hydrophobic cluster in their C-terminus.; Isoform PACE4E-II: Endomembrane system; Peripheral membrane protein. Retained intracellularly probably through a hydrophobic cluster in their C-terminus.; Isoform PACE4B: Secreted. |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.279% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1:500-2000 |
| Immunogen | Synthesized peptide derived from human PCSK6 AA range: 734-784 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Rat | — | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.