Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM94968

PDE6G Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

PDE6G Rabbit pAb from ELK Biotechnology Read more ›

PDE6G Rabbit pAb
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PDE6G Rabbit pAb

Product Description

PDE6G Rabbit pAb is a research-use antibody from ELK Biotechnology for studying PDE6G in IHC, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes the gamma subunit of cyclic GMP-phosphodiesterase, which is composed of alpha- and beta- catalytic subunits and two identical, inhibitory gamma subunits. This gene is expressed in rod photoreceptors and functions in the phototransduction signaling cascade. It is also expressed in a variety of other tissues, and has been shown to regulate the c-Src protein kinase and G-protein-coupled receptor kinase 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2009],

Additional antibody information

Specificity This antibody detects endogenous levels of human PDE6G
Validation evidence captions Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Key Facts

Product typePrimary Antibody Target: PDE6G
Collaborator ELK Biotechnology
Partner model ES14185
Target PDE6G
Research areas>>Purine metabolism, >>Metabolic pathways, >>Phototransduction
Reactivity Human,Mouse
Applications IHC,ELISA
ConjugationUnconjugated
Dilution rangeIHC-p 1:50-200, ELISA(peptide)1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human PDE6G AA range: 10-90
Concentration 1 mg/ml
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Human retina,Retina,
Purity The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
Human IHC-p 1:50-200
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
PDE6G
Also known as
Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma (GMP-PDE gamma;EC 3.1.4.35)
Concentration
1 mg/ml
Working concentration
IHC-p 1:50-200, ELISA(peptide)1:5000-20000
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
IHCELISA
Immunogen
Synthesized peptide derived from human PDE6G AA range: 10-90
Cellular localization
plasma membrane,photoreceptor disc membrane,
Dilution range
IHC-p 1:50-200, ELISA(peptide)1:5000-20000
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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