HDAC2 (phospho Ser394) rabbit pAb from ELK Biotechnology Read more ›
HDAC2 (phospho Ser394) rabbit pAb is a research-use antibody from ELK Biotechnology for studying HDAC2 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010],
| Specificity | Phospho-HDAC2 (S394) Polyclonal Antibody detects endogenous levels of HDAC2 protein only when phosphorylated at S394. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-HDAC2 (S394) Polyclonal Antibody | Western Blot analysis of 3T3 cells using Phospho-HDAC2 (S394) Polyclonal Antibody | Western blot analysis of lysates from LOVO cells, using HDAC2 (Phospho-Ser394) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1417 |
| Target | HDAC2 |
| Research areas | >>Cell cycle, >>Longevity regulating pathway - multiple species, >>Notch signaling pathway, >>Neutrophil extracellular trap formation, >>Thyroid hormone signaling pathway, >>Huntington disease, >>Amphetamine addiction, >>Alcoholism, >>Human papillomavirus infection, >>Epstein-Barr virus infection, >>Pathways in cancer, >>Transcriptional misregulation in cancer, >>Viral carcinogenesis, >>MicroRNAs in cancer, >>Chronic myeloid leukemia |
| Reactivity | Human,Mouse,Rat,Monkey |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HDAC2 around the phosphorylation site of Ser394. AA range:360-409 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Widely expressed; lower levels in brain and lung. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Monkey | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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