ABM94960
HDAC2 (phospho Ser394) rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
HDAC2 (phospho Ser394) rabbit pAb from ELK Biotechnology Read more ›
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Product Description
HDAC2 (phospho Ser394) rabbit pAb is an ELK Biotechnology antibody for research workflows involving HDAC2.
Background
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010],
Additional antibody information
| Specificity | Phospho-HDAC2 (S394) Polyclonal Antibody detects endogenous levels of HDAC2 protein only when phosphorylated at S394. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-HDAC2 (S394) Polyclonal Antibody | Western Blot analysis of 3T3 cells using Phospho-HDAC2 (S394) Polyclonal Antibody | Western blot analysis of lysates from LOVO cells, using HDAC2 (Phospho-Ser394) Antibody. The lane on the right is blocked with the phospho peptide. |
Key Facts
Product type
Primary Antibody›
Target: HDAC2
| Target | HDAC2 |
| Also known as | HDAC2; Histone deacetylase 2; HD2 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat;Monkey |
| Observed band | 55kD |
| Cellular localization | Nucleus . Cytoplasm . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Cell cycle;>>Longevity regulating pathway - multiple species;>>Notch signaling pathway;>>Neutrophil extracellular trap formation;>>Thyroid hormone signaling pathway;>>Huntington disease;>>Amphetamine addiction;>>Alcoholism;>>Human papillomavirus infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Viral carcinogenesis;>>MicroRNAs in cancer;>>Chronic myeloid leukemia |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HDAC2 around the phosphorylation site of Ser394. AA range:360-409 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
4 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Monkey |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Monkey | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
HDAC2
Also known as
HDAC2; Histone deacetylase 2; HD2
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat;Monkey
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human HDAC2 around the phosphorylation site of Ser394. AA range:360-409
Observed band
55kD
Cellular localization
Nucleus . Cytoplasm .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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