CaMKIIα/β/δ (phospho Thr305) rabbit pAb from ELK Biotechnology Read more ›
CaMKIIα/β/δ (phospho Thr305) rabbit pAb is a research-use antibody from ELK Biotechnology for studying CaMKIIα/β/δ in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008],
| Specificity | Phospho-CaMKIIα/β/δ (T305) Polyclonal Antibody detects endogenous levels of CaMKIIα/β/δ protein only when phosphorylated at T305. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-CaMKIIα/β/δ (T305) Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of 453 cells using Phospho-CaMKIIα/β/δ (T305) Polyclonal Antibody diluted at 1:1000 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CaMK2 alpha/beta/delta (Phospho-Thr305) Antibody | Immunohistochemistry analysis of paraffin-embedded human brain, using CaMK2 alpha/beta/delta (Phospho-Thr305) Antibody. The picture on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1415 |
| Target | CaMKIIα/β/δ |
| Research areas | >>ErbB signaling pathway, >>Calcium signaling pathway, >>cAMP signaling pathway, >>HIF-1 signaling pathway, >>Oocyte meiosis, >>Necroptosis, >>Adrenergic signaling in cardiomyocytes, >>Wnt signaling pathway, >>Axon guidance, >>Circadian entrainment, >>Long-term potentiation, >>Neurotrophin signaling pathway, >>Cholinergic synapse, >>Dopaminergic synapse, >>Olfactory transduction, >>Inflammatory mediator regulation of TRP channels, >>Insulin secretion, >>GnRH signaling pathway, >>Melanogenesis, >>Oxytocin signaling pathway, >>Glucagon signaling pathway, >>Aldosterone synthesis and secretion, >>Cushing syndrome, >>Gastric acid secretion, >>Parkinson disease, >>Pathways of neurodegeneration - multiple diseases, >>Amphetamine addiction, >>Tuberculosis, >>Pathways in cancer, >>Proteoglycans in cancer, >>Glioma, >>Diabetic cardiomyopathy, >>Lipid and atherosclerosis |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CaMK2 alpha/beta/delta around the phosphorylation site of Thr305. AA range:271-320 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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