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Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM94932

PI51A rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

PI51A rabbit pAb from ELK Biotechnology Read more ›

PI51A rabbit pAb
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PI51A rabbit pAb

Product Description

PI51A rabbit pAb is a research-use antibody from ELK Biotechnology for studying PI51A in WB workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Additional antibody information

Specificity This antibody detects endogenous levels of PI51A at Human/Mouse
Validation evidence captions Western blot analysis of lysates from AD293 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody› Target: PI51A
Collaborator ELK Biotechnology
Partner model ES14115
Target PI51A
Research areas>>Inositol phosphate metabolism, >>Metabolic pathways, >>Phosphatidylinositol signaling system, >>Phospholipase D signaling pathway, >>Endocytosis, >>Focal adhesion, >>Fc gamma R-mediated phagocytosis, >>Regulation of actin cytoskeleton, >>Yersinia infection, >>Choline metabolism in cancer
Reactivity Human,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human PI51A AA range: 270-320
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver.
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 —
Mouse — —

Specifications

Target
PI51A
Also known as
Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha (PIP5K1-alpha) (PtdIns(4)P-5-kinase 1 alpha) (EC 2.7.1.68) (68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha) (Phosphatidylinositol 4-phosphate 5-kinase type I alpha) (PIP5KIalpha)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human PI51A AA range: 270-320
Observed band
60kD
Cellular localization
Cell membrane . Cytoplasm . Nucleus . Nucleus speckle . Cell projection, ruffle . Cell projection, lamellipodium . Colocalizes with RAC1 at actin-rich membrane ruffles (PubMed:20660631). Localizes to nuclear speckles and associates with TUT1 to regulate polyadenylation of selected mRNAs (PubMed:18288197). .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.99% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified™

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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