Synapsin I (phospho Ser9) rabbit pAb
Synapsin I (phospho Ser9) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Synapsin I (phospho Ser9) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Synapsin I.
Background
This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | Phospho-Synapsin I (S9) Polyclonal Antibody detects endogenous levels of Synapsin I protein only when phosphorylated at S9. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-Synapsin I (S9) Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of 3T3 cells using Phospho-Synapsin I (S9) Polyclonal Antibody diluted at 1:1000 | Western blot analysis of HELA KB 293T 3T3 lysis using Phospho-Synapsin I (S9) antibody. Antibody was diluted at 1:1000 | Western blot analysis of lysates from 293 cells treated with PMA 200nM 30', using Synapsin (Phospho-Ser9) Antibody. The lane on the right is blocked with the phospho peptide. |
Key Facts
| Target | Synapsin I |
| Also known as | SYN1; Synapsin-1; Brain protein 4.1; Synapsin I |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 77kD |
| Cellular localization | Cell junction, synapse. Golgi apparatus . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Synapsin around the phosphorylation site of Ser9. AA range:3-52 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.