PLCγ2 (phospho-Tyr759) rabbit pAb from ELK Biotechnology Read more ›
PLCγ2 (phospho-Tyr759) rabbit pAb is a research-use antibody from ELK Biotechnology for studying PLCG2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. [provided by RefSeq, Mar 2014],
| Specificity | This antibody detects endogenous levels of Human Mouse PLCγ2 (phospho-Tyr759) |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES14068 |
| Target | PLCG2 |
| Research areas | >>Inositol phosphate metabolism, >>Metabolic pathways, >>EGFR tyrosine kinase inhibitor resistance, >>ErbB signaling pathway, >>Ras signaling pathway, >>Calcium signaling pathway, >>Chemokine signaling pathway, >>NF-kappa B signaling pathway, >>HIF-1 signaling pathway, >>Phosphatidylinositol signaling system, >>Phospholipase D signaling pathway, >>Axon guidance, >>VEGF signaling pathway, >>Osteoclast differentiation, >>Platelet activation, >>Neutrophil extracellular trap formation, >>C-type lectin receptor signaling pathway, >>Natural killer cell mediated cytotoxicity, >>B cell receptor signaling pathway, >>Fc epsilon RI signaling pathway, >>Fc gamma R-mediated phagocytosis, >>Leukocyte transendothelial migration, >>Neurotrophin signaling pathway, >>Inflammatory mediator regulation of TRP channels, >>Thyroid hormone signaling pathway, >>AGE-RAGE signaling pathway in diabetic complications, >>Growth hormone synthesis, secretion and action, >>Vibrio cholerae infection, >>Epithelial cell signaling in Helicobacter py |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000; IF/ICC 1:50-200;ELISA 1:2000-20000;IHC-p 1:50-200 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized phosho peptide around human PLCγ2 (Tyr759) |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Lymph,Lymphoblast,Spleen,T-cell, |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000; IF/ICC 1:50-200;ELISA 1:2000-20000;IHC-p 1:50-200 | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
