ABM94910
PLCγ2 (phospho-Tyr759) rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
PLCγ2 (phospho-Tyr759) rabbit pAb from ELK Biotechnology Read more ›
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Product Description
PLCγ2 (phospho-Tyr759) rabbit pAb is an ELK Biotechnology antibody for research workflows involving PLCG2.
Background
The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. [provided by RefSeq, Mar 2014],
Additional antibody information
| Specificity | This antibody detects endogenous levels of Human Mouse PLCγ2 (phospho-Tyr759) |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
Product type
Primary Antibody›
Target: PLCG2
| Target | PLCG2 |
| Also known as | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 (EC 3.1.4.11) (Phosphoinositide phospholipase C-gamma-2) (Phospholipase C-IV) (PLC-IV) (Phospholipase C-gamma-2) (PLC-gamma-2) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000; IF/ICC 1:50-200;ELISA 1:2000-20000;IHC-p 1:50-200 |
| Species reactivity | Human;Mouse |
| Observed band | 148kD |
| Cellular localization | intracellular,cytosol,plasma membrane,extracellular exosome, |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Inositol phosphate metabolism;>>Metabolic pathways;>>EGFR tyrosine kinase inhibitor resistance;>>ErbB signaling pathway;>>Ras signaling pathway;>>Calcium signaling pathway;>>Chemokine signaling pathway;>>NF-kappa B signaling pathway;>>HIF-1 signaling pathway;>>Phosphatidylinositol signaling system;>>Phospholipase D signaling pathway;>>Axon guidance;>>VEGF signaling pathway;>>Osteoclast differentiation;>>Platelet activation;>>Neutrophil extracellular trap formation;>>C-type lectin receptor signaling pathway;>>Natural killer cell mediated cytotoxicity;>>B cell receptor signaling pathway;>>Fc epsilon RI signaling pathway;>>Fc gamma R-mediated phagocytosis;>>Leukocyte transendothelial migration;>>Neurotrophin signaling pathway;>>Inflammatory mediator regulation of TRP channels;>>Thyroid hormone signaling pathway;>>AGE-RAGE signaling pathway in diabetic complications;>>Growth hormone synthesis, secretion and action;>>Vibrio cholerae infection;>>Epithelial cell signaling in Helicobacter py |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | WB 1:500-2000; IF/ICC 1:50-200;ELISA 1:2000-20000;IHC-p 1:50-200 |
| Immunogen | Synthesized phosho peptide around human PLCγ2 (Tyr759) |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
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Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000; IF/ICC 1:50-200;ELISA 1:2000-20000;IHC-p 1:50-200 | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
PLCG2
Also known as
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 (EC 3.1.4.11) (Phosphoinositide phospholipase C-gamma-2) (Phospholipase C-IV) (PLC-IV) (Phospholipase C-gamma-2) (PLC-gamma-2)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000; IF/ICC 1:50-200;ELISA 1:2000-20000;IHC-p 1:50-200
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse
Tested applications
WBIHCIFELISA
Immunogen
Synthesized phosho peptide around human PLCγ2 (Tyr759)
Observed band
148kD
Cellular localization
intracellular,cytosol,plasma membrane,extracellular exosome,
Dilution range
WB 1:500-2000; IF/ICC 1:50-200;ELISA 1:2000-20000;IHC-p 1:50-200
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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