Stat3 (phospho Ser727) rabbit pAb from ELK Biotechnology Read more ›
Stat3 (phospho Ser727) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Stat3 in WB, IHC, IF, ELISA, IP workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper
| Specificity | Phospho-Stat3 (S727) Polyclonal Antibody detects endogenous levels of Stat3 protein only when phosphorylated at S727. |
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| Collaborator | ELK Biotechnology |
| Partner model | ES1405 |
| Target | Stat3 |
| Research areas | >>EGFR tyrosine kinase inhibitor resistance, >>Chemokine signaling pathway, >>HIF-1 signaling pathway, >>FoxO signaling pathway, >>Necroptosis, >>Signaling pathways regulating pluripotency of stem cells, >>JAK-STAT signaling pathway, >>Th17 cell differentiation, >>Prolactin signaling pathway, >>Adipocytokine signaling pathway, >>Insulin resistance, >>AGE-RAGE signaling pathway in diabetic complications, >>Growth hormone synthesis, secretion and action, >>Toxoplasmosis, >>Hepatitis C, >>Hepatitis B, >>Measles, >>Human cytomegalovirus infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Epstein-Barr virus infection, >>Coronavirus disease - COVID-19, >>Pathways in cancer, >>Viral carcinogenesis, >>Proteoglycans in cancer, >>MicroRNAs in cancer, >>Chemical carcinogenesis - receptor activation, >>Pancreatic cancer, >>Acute myeloid leukemia, >>Non-small cell lung cancer, >>PD-L1 expression and PD-1 checkpoint pathway in cancer, >>Inflammatory bowel disease, >>Lipid and atherosclerosis |
| Reactivity | Human,Mouse,Rat,Monkey |
| Applications | WB,IHC,IF,ELISA,IP |
| Conjugation | Unconjugated |
| Dilution range | IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human STAT3 around the phosphorylation site of Ser727. AA range:694-743 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in naive CD4(+) T cells as well as T-helper Th17, Th1 and Th2 cells (PubMed:31899195). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
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| Human | |||||||
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| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Monkey | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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