Primary Antibody ISO 9001|ABMIUM Verified™
ABM94880

POMT2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

POMT2 rabbit pAb from ELK Biotechnology Read more ›

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POMT2 rabbit pAb
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POMT2 rabbit pAb

Product Description

POMT2 rabbit pAb is an ELK Biotechnology antibody for research workflows involving POMT2.

Background

The protein encoded by this gene is an O-mannosyltransferase that requires interaction with the product of the POMT1 gene for enzymatic function. The encoded protein is found in the membrane of the endoplasmic reticulum. Defects in this gene are a cause of Walker-Warburg syndrome (WWS).[provided by RefSeq, Oct 2008],

Additional antibody information

Specificity This antibody detects endogenous levels of POMT2 at Human/Mouse
Validation evidence captions Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°over night | Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product type Primary Antibody Target: POMT2
Target POMT2
Concentration 1 mg/ml
Working concentration WB 1:500-2000;IHC-p 1:50-300
Species reactivity Human; Mouse
Cellular localization Endoplasmic reticulum membrane ; Multi-pass membrane protein .
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Research area >>Other types of O-glycan biosynthesis;>>Mannose type O-glycan biosynthesis;>>Metabolic pathways
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Conjugation Unconjugated
Tested applications
WBIHC
Dilution range WB 1:500-2000;IHC-p 1:50-300
Immunogen Synthesized peptide derived from human POMT2 AA range: 177-227
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Reactivity & Application Validation

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMCHIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
POMT2
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human; Mouse
Tested applications
WBIHC
Immunogen
Synthesized peptide derived from human POMT2 AA range: 177-227
Cellular localization
Endoplasmic reticulum membrane ; Multi-pass membrane protein .
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ISO 9001
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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