Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM94802

PTHD3 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

PTHD3 rabbit pAb from ELK Biotechnology Read more ›

PTHD3 rabbit pAb
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PTHD3 rabbit pAb

Product Description

PTHD3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying PTHD3 in WB workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Additional antibody information

Specificity This antibody detects endogenous levels of PTHD3 at Human/Mouse
Validation evidence captions Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: PTHD3
Collaborator ELK Biotechnology
Partner model ES13864
Target PTHD3
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human PTHD3 AA range: 244-294
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in germ cells of the testis (at protein level) (PubMed:17904097). Detected in blood lymph, colon, small intestine, ovary, testis, prostate, thymus and spleen with highest levels in testis (PubMed:21439084).
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse

Specifications

Target
PTHD3
Also known as
Patched domain-containing protein 3 (Patched-related protein)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human PTHD3 AA range: 244-294
Observed band
85kD
Cellular localization
Cell projection, cilium, flagellum membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Localizes to the midpiece of the sperm tail. .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.322% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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