NFκB-p65 (phospho Ser529) rabbit pAb from ELK Biotechnology Read more ›
NFκB-p65 (phospho Ser529) rabbit pAb is a research-use antibody from ELK Biotechnology for studying NFkB p65 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],
| Specificity | Phospho-NFκB-p65 (S529) Polyclonal Antibody detects endogenous levels of NFκB-p65 protein only when phosphorylated at S529. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-NFκB-p65 (S529) Polyclonal Antibody diluted at 1:2000 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NF-kappaB p65 (Phospho-Ser529) Antibody | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 (Phospho-Ser529) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from HeLa and Jurkat cells, using NF-kappaB p65 (Phospho-Ser529) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1371 |
| Target | NFkB p65 |
| Research areas | >>Antifolate resistance, >>MAPK signaling pathway, >>Ras signaling pathway, >>cAMP signaling pathway, >>Chemokine signaling pathway, >>NF-kappa B signaling pathway, >>HIF-1 signaling pathway, >>Sphingolipid signaling pathway, >>Mitophagy - animal, >>PI3K-Akt signaling pathway, >>Apoptosis, >>Longevity regulating pathway, >>Cellular senescence, >>Osteoclast differentiation, >>Neutrophil extracellular trap formation, >>Toll-like receptor signaling pathway, >>NOD-like receptor signaling pathway, >>RIG-I-like receptor signaling pathway, >>Cytosolic DNA-sensing pathway, >>C-type lectin receptor signaling pathway, >>IL-17 signaling pathway, >>Th1 and Th2 cell differentiation, >>Th17 cell differentiation, >>T cell receptor signaling pathway, >>B cell receptor signaling pathway, >>TNF signaling pathway, >>Neurotrophin signaling pathway, >>Prolactin signaling pathway, >>Adipocytokine signaling pathway, >>Relaxin signaling pathway, >>Insulin resistance, >>Non-alcoholic fatty liver disease, >>AGE-RAGE signaling pathway in diabe |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Ser529. AA range:496-545 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Bone,Colon,Pancreas,Placenta, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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