Met (phospho Tyr1349) rabbit pAb
Met (phospho Tyr1349) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Met (phospho Tyr1349) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Met.
Background
This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. [provided by RefSeq, May 2016],
Additional antibody information
| Specificity | Phospho-Met (Y1349) Polyclonal Antibody detects endogenous levels of Met protein only when phosphorylated at Y1349. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-Met (Y1349) Polyclonal Antibody diluted at 1:1000 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Met (Phospho-Tyr1349) Antibody | Western blot analysis of lysates from HepG2 cells, using Met (Phospho-Tyr1349) Antibody. The lane on the right is blocked with the phospho peptide. | Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
| Target | Met |
| Also known as | MET; Hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 155kD |
| Cellular localization | Membrane; Single-pass type I membrane protein.; Isoform 3: Secreted. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>EGFR tyrosine kinase inhibitor resistance;>>MAPK signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>Calcium signaling pathway;>>PI3K-Akt signaling pathway;>>Axon guidance;>>Focal adhesion;>>Adherens junction;>>Bacterial invasion of epithelial cells;>>Epithelial cell signaling in Helicobacter pylori infection;>>Malaria;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Proteoglycans in cancer;>>MicroRNAs in cancer;>>Chemical carcinogenesis - reactive oxygen species;>>Renal cell carcinoma;>>Melanoma;>>Non-small cell lung cancer;>>Hepatocellular carcinoma;>>Gastric cancer;>>Central carbon metabolism in cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCELISA |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Met around the phosphorylation site of Tyr1349. AA range:1316-1365 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.