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Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM94686

RN112 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

RN112 rabbit pAb from ELK Biotechnology Read more ›

RN112 rabbit pAb
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RN112 rabbit pAb

Product Description

RN112 rabbit pAb is a research-use antibody from ELK Biotechnology for studying RN112 in WB, IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the RING finger protein family of transcription factors. The protein is primarily expressed in brain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity This antibody detects endogenous levels of RN112 at Human/Mouse/Rat
Validation evidence captions Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night | Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product typePrimary Antibody› Target: RN112
Collaborator ELK Biotechnology
Partner model ES13376
Target RN112
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:500-2000;IHC-p 1:50-300
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human RN112 AA range: 377-427
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Predominantly expressed in brain (PubMed:10574464). Decreased expression in glioma brain tumors as compared to normal brains (at protein level) (PubMed:28684796).
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300 —
Mouse — —
Rat — —
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
RN112
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBIHC
Immunogen
Synthesized peptide derived from human RN112 AA range: 377-427
Cellular localization
Membrane ; Multi-pass membrane protein . Membrane ; Peripheral membrane protein . Cytoplasm . Nucleus . Nucleus, nuclear body . Nucleus, nucleoplasm . Endosome . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle . Cell junction, synapse, postsynaptic density . Perikaryon . Cell projection, neuron projection . Predominantly in the nucleus, but some amounts were also found in the cytoplasm. Oxidative stress stimulates its shuttling from the cytoplasm into the nucleus. Recruited to nuclear bodies via its interaction with ZBTB16. Localizes to the cell soma and neuritis and only slightly to the nucleus in the neurons of most brain areas. .
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified™

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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