ABM94660
RPAB4 Rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
RPAB4 Rabbit pAb from ELK Biotechnology Read more ›
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Product Description
RPAB4 Rabbit pAb is an ELK Biotechnology antibody for research workflows involving RPAB4.
Background
This gene encodes one of the smallest subunits of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | This antibody detects endogenous levels of human RPAB4 |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
Product type
Primary Antibody›
Target: RPAB4
| Target | RPAB4 |
| Also known as | DNA-directed RNA polymerases I, II, and III subunit RPABC4 (RNA polymerases I, II, and III subunit ABC4;ABC10-alpha;DNA-directed RNA polymerase II subunit K;RNA polymerase II 7.0 kDa subunit;RPB7.0;RPB10alpha) |
| Concentration | 1 mg/ml |
| Working concentration | IHC-p 1:50-200, ELISA(peptide)1:5000-20000 |
| Species reactivity | Human; Mouse |
| Cellular localization | Nucleus . |
| Purity | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>RNA polymerase;>>Cytosolic DNA-sensing pathway;>>Huntington disease |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
IHCELISA |
| Dilution range | IHC-p 1:50-200, ELISA(peptide)1:5000-20000 |
| Immunogen | Synthesized peptide derived from human RPAB4 AA range: 1-50 |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
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Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC-p 1:50-200 | — |
| Mouse | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
RPAB4
Also known as
DNA-directed RNA polymerases I, II, and III subunit RPABC4 (RNA polymerases I, II, and III subunit ABC4;ABC10-alpha;DNA-directed RNA polymerase II subunit K;RNA polymerase II 7.0 kDa subunit;RPB7.0;RPB10alpha)
Concentration
1 mg/ml
Working concentration
IHC-p 1:50-200, ELISA(peptide)1:5000-20000
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human; Mouse
Tested applications
IHCELISA
Immunogen
Synthesized peptide derived from human RPAB4 AA range: 1-50
Cellular localization
Nucleus .
Dilution range
IHC-p 1:50-200, ELISA(peptide)1:5000-20000
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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