HDAC5 (phospho Ser498) rabbit pAb from ELK Biotechnology Read more ›
HDAC5 (phospho Ser498) rabbit pAb is a research-use antibody from ELK Biotechnology for studying HDAC5 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-HDAC5 (S498) Polyclonal Antibody detects endogenous levels of HDAC5 protein only when phosphorylated at S498. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-HDAC5 (S498) Polyclonal Antibody diluted at 1:500 | Western Blot analysis of VEC cells using Phospho-HDAC5 (S498) Polyclonal Antibody diluted at 1:500 | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HDAC5 (Phospho-Ser498) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from Jurkat cells, using HDAC5 (Phospho-Ser498) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1324 |
| Target | HDAC5 |
| Research areas | >>Apelin signaling pathway, >>Neutrophil extracellular trap formation, >>Alcoholism, >>Viral carcinogenesis, >>MicroRNAs in cancer |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HDAC5 around the phosphorylation site of Ser498. AA range:464-513 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Ubiquitous. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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