Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM94576

GSK3β (phospho Ser9) rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

GSK3β (phospho Ser9) rabbit pAb from ELK Biotechnology Read more ›

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Product Description

GSK3β (phospho Ser9) rabbit pAb is a research-use antibody from ELK Biotechnology for studying GSK3β in WB, IHC, IF, ELISA, IP workflows with listed reactivity for Human, Mouse, Rat, Drosophila. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009],

Additional antibody information

Specificity Phospho-GSK3β (S9) Polyclonal Antibody detects endogenous levels of GSK3β protein only when phosphorylated at S9.

Key Facts

Product typePrimary Antibody Target: GSK3β
Collaborator ELK Biotechnology
Partner model ES1322
Target GSK3β
Research areas>>EGFR tyrosine kinase inhibitor resistance, >>ErbB signaling pathway, >>Chemokine signaling pathway, >>Cell cycle, >>mTOR signaling pathway, >>PI3K-Akt signaling pathway, >>Wnt signaling pathway, >>Hedgehog signaling pathway, >>Axon guidance, >>Hippo signaling pathway, >>Focal adhesion, >>Signaling pathways regulating pluripotency of stem cells, >>IL-17 signaling pathway, >>T cell receptor signaling pathway, >>B cell receptor signaling pathway, >>Neurotrophin signaling pathway, >>Dopaminergic synapse, >>Insulin signaling pathway, >>Melanogenesis, >>Prolactin signaling pathway, >>Thyroid hormone signaling pathway, >>Insulin resistance, >>Non-alcoholic fatty liver disease, >>Cushing syndrome, >>Growth hormone synthesis, secretion and action, >>Alcoholic liver disease, >>Alzheimer disease, >>Prion disease, >>Pathways of neurodegeneration - multiple diseases, >>Shigellosis, >>Yersinia infection, >>Hepatitis C, >>Measles, >>Human cytomegalovirus infection, >>Human papillomavirus infection, >>Kaposi sarcoma-associated herpes
Reactivity Human,Mouse,Rat,Drosophila
Applications WB,IHC,IF,ELISA,IP
ConjugationUnconjugated
Dilution rangeIF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/5000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human GSK3 beta around the phosphorylation site of Ser9. AA range:1-50
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. Colocalizes with EIF2AK2/PKR and TAU in the Alzheimer disease (AD) brain.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

4 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Drosophila
ABMIUM Validated™ WB
Species Dilution Notes
Human IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/5000. Not yet tested in other applications.
Mouse
Rat
Drosophila
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes
ABMIUM Validated™ IP
Species Dilution Notes

Specifications

Target
GSK3β
Also known as
GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B
Concentration
1 mg/ml
Working concentration
IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat,Drosophila
Tested applications
WBIHCIFELISAIP
Immunogen
The antiserum was produced against synthesized peptide derived from human GSK3 beta around the phosphorylation site of Ser9. AA range:1-50
Observed band
48kD
Cellular localization
Cytoplasm . Nucleus . Cell membrane . The phosphorylated form shows localization to cytoplasm and cell membrane (PubMed:20937854). The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane (PubMed:20937854). .
Dilution range
IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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