Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM94565

SAMHD1 (phospho-Thr592) rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

SAMHD1 (phospho-Thr592) rabbit pAb from ELK Biotechnology Read more ›

SAMHD1 (phospho-Thr592) rabbit pAb
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SAMHD1 (phospho-Thr592) rabbit pAb

Product Description

SAMHD1 (phospho-Thr592) rabbit pAb is a research-use antibody from ELK Biotechnology for studying SAMHD1 in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1(SAMHD1) Homo sapiens This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome. [provided by RefSeq, Mar 2010],

Additional antibody information

Specificity This antibody detects endogenous levels of Human Mouse Rat SAMHD1 (phospho-Thr592)
Validation evidence captions Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000

Key Facts

Product typePrimary Antibody Target: SAMHD1
Collaborator ELK Biotechnology
Partner model ES13204
Target SAMHD1
Research areas>>Viral life cycle - HIV-1, >>Human immunodeficiency virus 1 infection
Reactivity Human,Mouse,Rat
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:1000-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized phosho peptide around human SAMHD1 (Thr592)
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in heart, skeletal muscle, spleen, liver, small intestine, placenta, lung and peripheral blood leukocytes (PubMed:11064105). No expression is seen in brain and thymus (PubMed:11064105).
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1000-2000
Mouse
Rat

Specifications

Target
SAMHD1
Also known as
SAM domain and HD domain-containing protein 1 (EC 3.1.4.-) (Dendritic cell-derived IFNG-induced protein) (DCIP) (Monocyte protein 5) (MOP-5)
Concentration
1 mg/ml
Working concentration
WB 1:1000-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WB
Immunogen
Synthesized phosho peptide around human SAMHD1 (Thr592)
Observed band
72kD
Cellular localization
Nucleus . Chromosome . Localizes to sites of DNA double-strand breaks in response to DNA damage. .
Dilution range
WB 1:1000-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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