Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM94557

SC5AC rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

SC5AC rabbit pAb from ELK Biotechnology Read more ›

SC5AC rabbit pAb
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SC5AC rabbit pAb

Product Description

SC5AC rabbit pAb is a research-use antibody from ELK Biotechnology for studying SC5AC in WB workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Normal blood lactate is maintained at about 1.5 mM, and little filtered lactate is excreted in urine. Reabsorption of lactate is mediated by the low-affinity Na(+)-coupled lactate transporter SLC5A12 in the initial part of the proximal tubule and by the high-affinity Na(+)-coupled lactate transporter SLC5A8 (MIM 608044) in the distal proximal tubule (Gopal et al., 2007 [PubMed 17692818]).[supplied by OMIM, Dec 2008],

Additional antibody information

Specificity This antibody detects endogenous levels of SC5AC at Human/Mouse
Validation evidence captions Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: SC5AC
Collaborator ELK Biotechnology
Partner model ES13190
Target SC5AC
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human SC5AC AA range: 84-134
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse

Specifications

Target
SC5AC
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human SC5AC AA range: 84-134
Cellular localization
Apical cell membrane ; Multi-pass membrane protein . Detected at the brush border membrane of the kidney. Colocalizes with viementin in Mueller cells (By similarity). .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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