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Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM94531

SEM4F rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

SEM4F rabbit pAb from ELK Biotechnology Read more ›

SEM4F rabbit pAb
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SEM4F rabbit pAb

Product Description

SEM4F rabbit pAb is a research-use antibody from ELK Biotechnology for studying SEM4F in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a transmembrane class IV semaphorin family protein, which plays a role in neural development. This gene may be involved in neurogenesis in prostate cancer, the development of neurofibromas, and breast cancer tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012],

Additional antibody information

Specificity This antibody detects endogenous levels of SEM4F at Human/Mouse/Rat
Validation evidence captions Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody› Target: SEM4F
Collaborator ELK Biotechnology
Partner model ES13160
Target SEM4F
Research areas>>Axon guidance
Reactivity Human,Mouse,Rat
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human SEM4F AA range: 85-135
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Amygdala,Brain,Liver,
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 —
Mouse — —
Rat — —

Specifications

Target
SEM4F
Also known as
Semaphorin-4F (Semaphorin-M) (Sema M) (Semaphorin-W) (Sema W)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WB
Immunogen
Synthesized peptide derived from human SEM4F AA range: 85-135
Observed band
85kD
Cellular localization
Cell membrane ; Single-pass type I membrane protein . Cell junction, synapse, postsynaptic density . Perikaryon . Cell projection, dendrite . Colocalizes with DLG4 at synapses. .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.336% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified™

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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